1HG8

Endopolygalacturonase from the phytopathogenic fungus Fusarium moniliforme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	58.260, 61.590, 98.860
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	17.900 - 1.730
R-factor	0.1728 *
Rwork	0.173
R-free	0.20300
Structure solution method	MIRAS
RMSD bond length	0.009
RMSD bond angle	2.195 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	17.900
High resolution limit [Å]	1.730
Rmerge	0.059	0.302 *
Total number of observations	204193 *
Number of reflections	37710
Completeness [%]	99.4	96.6 *
Redundancy	4.56

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.7	294 *	Federici, L., (2001) Acta Crystallogr.,Sect.D, 55, 1359. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG4000	37-42 (%(w/v))
3	1	reservoir	sodium citrate	0.1 (M)	pH4.7