1HG4

Ultraspiracle ligand binding domain from Drosophila melanogaster

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 1
Unit cell lengths	37.980, 86.190, 137.000
Unit cell angles	85.57, 85.94, 83.09

Refinement procedure

Resolution	34.000 - 2.400
R-factor	0.248
Rwork	0.248
R-free	0.28400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ere
RMSD bond length	0.008
RMSD bond angle	1.200
Data reduction software	MOSFLM
Phasing software	CNS (1.0)
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.000
High resolution limit [Å]	2.200	2.400 *
Rmerge	0.126	0.305 *
Total number of observations	175324 *
Number of reflections	60384
<I/σ(I)>	4.4
Completeness [%]	90.0
Redundancy	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	4.6 *	18 *	used streak-seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	ammonium sulfate	130 (mM)
3	1	reservoir	sodium acetate	160 (mM)
4	1	reservoir	PEG2000	25 (%)