1HFY

ALPHA-LACTALBUMIN

Experimental procedure

Temperature [K]	298
Collection date	1994-05-01
Detector	SIEMENS
Spacegroup name	P 1 21 1
Unit cell lengths	32.500, 89.700, 45.200
Unit cell angles	90.00, 94.50, 90.00

Refinement procedure

Resolution	8.000 - 2.300
R-factor	0.195
Rwork	0.195
R-free	0.27000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	HUMAN ALPHA-LACTALBUMIN
RMSD bond length	0.011
RMSD bond angle	23.400 *
Data reduction software	XDS
Data scaling software	XDS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	32.200
High resolution limit [Å]	2.300
Rmerge	0.096
Number of reflections	10415
Completeness [%]	90.5
Redundancy	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.5	16 *	0.2M AMMONIUM SULFATE, 20-25% (W/V) PEG 4000, 0.1M SODIUM ACETATE PH4.5, 20MG/ML PROTEIN 16 DEGREES C, temperature 289K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium salfate	0.2 (M)
2	1	reservoir	PEG4000	20-25 (%)
3	1	reservoir		0.1 (M)
4	1	drop	protein	20 (mg/ml)