1HFO
The Structure of the Macrophage Migration Inhibitory Factor from Trichinella Spiralis.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-11-15 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 110.128, 88.344, 86.355 |
| Unit cell angles | 90.00, 131.13, 90.00 |
Refinement procedure
| Resolution | 28.330 - 1.650 |
| R-factor | 0.225 |
| Rwork | 0.225 |
| R-free | 0.28100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mif |
| RMSD bond length | 0.008 |
| RMSD bond angle | 24.900 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.057 * | 0.281 * |
| Total number of observations | 163895 * | |
| Number of reflections | 72915 | 7060 * |
| <I/σ(I)> | 3.6 | 2.5 |
| Completeness [%] | 97.6 | 97.6 |
| Redundancy | 2.3 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 4.6 | CRYSTALS GROWN BY HANGING DROP METHOD. WELL CONTAINED 400-600 AMMONIUM ACETATE, 30-36% PEG4K, 100MM SODIUM ACETATE PH4.6. DROP CONTAINED 1:1 MIX OF WELL AND 16.5MG/ML TSMIF IN MINIMAL BUFFER., pH 4.60 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 16.5 (mg/ml) | |
| 2 | 1 | 1 | ammonium acetate | 400-600 (mM) | |
| 3 | 1 | 1 | PEG4000 | 30-36 (%) | |
| 4 | 1 | 1 | sodium acetate | 100 (mM) |
