1HF8

CALM-N N-terminal domain of clathrin assembly lymphoid myeloid leukaemia protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	293
Detector technology	CCD
Collection date	2000-02-02
Detector	ADSC CCD
Spacegroup name	P 41 21 2
Unit cell lengths	77.929, 77.929, 121.854
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.940 - 2.000
R-factor	0.19207 *
Rwork	0.190
R-free	0.22252 *
Structure solution method	SIRAS
RMSD bond length	0.031 *
RMSD bond angle	2.100 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.000	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.080	1.036
Number of reflections	26116
<I/σ(I)>	27.8	2.8
Completeness [%]	100.0	100
Redundancy	14	13.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5		0.1M HEPES, PH 7.5, 12% PEG 8K, 8% ETHYLENE GLYCOL

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	HEPES	0.1 (M)
2	1	reservoir	PEG8000	12-14 (%)
3	1	reservoir	ethylene glycol	8 (%)