1HF2

Crystal structure of the bacterial cell-division inhibitor MinC from T. maritima

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	51.580, 106.090, 162.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	100.000 - 2.200
R-factor	0.237
Rwork	0.237
R-free	0.30010
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.279
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.075	0.263
Number of reflections	40403
<I/σ(I)>	13.6	3.7
Completeness [%]	95.0	87.3
Redundancy	3.2	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	5 *		pH 6.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG2000	21 (%)
2	1	reservoir	sodium citrate	0.1 (M)
3	1	reservoir	ethanol	5.8 (%)
4	1	drop	protein	10 (mg/ml)