1HEY

INVESTIGATING THE STRUCTURAL DETERMINANTS OF THE P21-LIKE TRIPHOSPHATE AND MG2+ BINDING SITE

Experimental procedure

Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X31
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X31
Detector technology	IMAGE PLATE
Collection date	1993-10-11
Detector	MARRESEARCH
Spacegroup name	P 65 2 2
Unit cell lengths	60.510, 60.510, 129.560
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.240
R-factor	0.2
Rwork	0.200
RMSD bond length	0.015
RMSD bond angle	1.800
Data reduction software	XDS
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.240 *	2.240 *
Rmerge	0.111
Total number of observations	74759 *
Number of reflections	6707
Completeness [%]	90.7	84.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	40 (mg/ml)
2	1	drop	Tris-HCl	5 (mM)
3	1	drop	PEG4000	30 (%(w/v))	precipitant
4	1	drop		0.2 (M)	precipitant
5	1	drop	Tris-HCl	0.1 (M)	precipitant