1HES
MU2 ADAPTIN SUBUNIT (AP50) OF AP2 ADAPTOR (SECOND DOMAIN), COMPLEXED WITH P-selectin INTERNALIZATION PEPTIDE SHLGTYGVFTNAA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-02-18 |
| Detector | ADSC CCD |
| Spacegroup name | P 64 |
| Unit cell lengths | 125.720, 125.720, 75.270 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 100.000 - 3.000 |
| R-factor | 0.213 |
| Rwork | 0.210 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bw8 |
| RMSD bond length | 0.030 * |
| RMSD bond angle | 2.348 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.000 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.146 | 0.011 |
| Number of reflections | 13729 | |
| <I/σ(I)> | 17.4 | 2 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 9.2 | 14.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 16 * | Owen, D.J., (1998) Science, 282, 1327. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12 (mg/ml) | |
| 2 | 1 | reservoir | 2.2 (M) | ||
| 3 | 1 | reservoir | sodium potassium phosphate | 0.4 (M) | |
| 4 | 1 | reservoir | dithiothreitol | 10 (mM) | |
| 5 | 1 | reservoir | glycerol | 15 (%(v/v)) | |
| 6 | 1 | reservoir | MES | 0.1 (M) |
