1HEI

STRUCTURE OF THE HEPATITIS C VIRUS RNA HELICASE DOMAIN

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	95
Detector technology	IMAGE PLATE
Collection date	1996-02-10
Detector	RIGAKU
Spacegroup name	P 21 21 21
Unit cell lengths	81.540, 102.730, 119.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.100
R-factor	0.22
Rwork	0.220
R-free	0.32000
Structure solution method	MIRAS
RMSD bond length	0.010
RMSD bond angle	23.740 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.052 *	0.270 *
Total number of observations	676708 *
Number of reflections	51694
<I/σ(I)>	15	3
Completeness [%]	88.0	70 *
Redundancy	12

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5 *	4 *	50MM CA ACETATE, 20MM NACACODYLATE PH 6.5, 8% PEG5000

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop	sodium HEPES	10 (mM)	pH7.5
3	1	drop	EDTA	1 (mM)
4	1	drop	dithiothreitol	5 (mM)
5	1	reservoir	cacodylate acetate	50 (mM)
6	1	reservoir	sodium cacodylate	20 (mM)	pH6.5
7	1	reservoir	PEG5000	8 (%)