1HEI
STRUCTURE OF THE HEPATITIS C VIRUS RNA HELICASE DOMAIN
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-02-10 |
| Detector | RIGAKU |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 81.540, 102.730, 119.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.100 |
| R-factor | 0.22 |
| Rwork | 0.220 |
| R-free | 0.32000 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 23.740 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.052 * | 0.270 * |
| Total number of observations | 676708 * | |
| Number of reflections | 51694 | |
| <I/σ(I)> | 15 | 3 |
| Completeness [%] | 88.0 | 70 * |
| Redundancy | 12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 * | 4 * | 50MM CA ACETATE, 20MM NACACODYLATE PH 6.5, 8% PEG5000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | sodium HEPES | 10 (mM) | pH7.5 |
| 3 | 1 | drop | EDTA | 1 (mM) | |
| 4 | 1 | drop | dithiothreitol | 5 (mM) | |
| 5 | 1 | reservoir | cacodylate acetate | 50 (mM) | |
| 6 | 1 | reservoir | sodium cacodylate | 20 (mM) | pH6.5 |
| 7 | 1 | reservoir | PEG5000 | 8 (%) |
