1HEE
Crystal structure of bovine pancreatic carboxypeptidase A complexed with L-N-hydroxyaminocarbonyl phenylalanine at 2.3 A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Temperature [K] | 297 |
Spacegroup name | P 1 |
Unit cell lengths | 65.570, 60.524, 74.410 |
Unit cell angles | 90.00, 97.84, 90.00 |
Refinement procedure
Resolution | 100.000 - 1.750 |
R-factor | 0.198 |
Rwork | 0.198 |
R-free | 0.22900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.254 * |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | |
High resolution limit [Å] | 1.750 | 1.800 * |
Rmerge | 0.048 | 0.179 * |
Number of reflections | 105084 | |
<I/σ(I)> | 15 | |
Completeness [%] | 90.3 | 77 * |
Redundancy | 1.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microdialysis * | 7.5 | pH 7.50 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 10 (mg/ml) | |
2 | 1 | 1 | Tris-HCl | 0.02 (M) | pH7.5 |
3 | 1 | 1 | 1.2 (M) | ||
4 | 1 | 2 | 0.14 (M) | pH7.5 |