1HDG

THE CRYSTAL STRUCTURE OF HOLO-GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE FROM THE HYPERTHERMOPHILIC BACTERIUM THERMOTOGA MARITIMA AT 2.5 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 61 2 2
Unit cell lengths	95.700, 95.700, 296.280
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.500
R-factor	0.166
Rwork	0.166
RMSD bond length	0.010
RMSD bond angle	1.770
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *
High resolution limit [Å]	2.500 *	2.500 *
Rmerge	0.054 *	0.139 *
Total number of observations	128503 *
Number of reflections	28069
Completeness [%]	99.7	41.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	4.6 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	1.8 (M)
2	1	drop	GAPDH	5.5 (mg/ml)
3	1	drop	ammonium sulfate	0.9 (M)
4	1	drop	NAD+	1.5 (mM)
5	1	drop	sodium acetate	0.1 (M)