1HDD
CRYSTAL STRUCTURE OF AN ENGRAILED HOMEODOMAIN-DNA COMPLEX AT 2.8 ANGSTROMS RESOLUTION: A FRAMEWORK FOR UNDERSTANDING HOMEODOMAIN-DNA INTERACTIONS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Detector technology | AREA DETECTOR |
| Detector | XENTRONICS |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 131.200, 45.500, 72.900 |
| Unit cell angles | 90.00, 119.00, 90.00 |
Refinement procedure
| Resolution | 10.001 - 2.800 |
| R-factor | 0.225 |
| Rwork | 0.225 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.590 |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.800 |
| Rmerge | 0.043 * |
| Total number of observations | 21130 * |
| Number of reflections | 9072 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6.7 | pH 6.70, VAPOR DIFFUSION |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | BIS-TRIS-PROPANE_HCL | ||
| 3 | 1 | 1 | NH4OH |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | DNA | double | ||
| 2 | 1 | 2 | Bis-Tris-HCl | 30 (mM) | |
| 3 | 1 | 1 | homeodomain | ||
| 4 | 1 | 1 | ammonium hydroxide |
