1HD5
Endoglucanase from Humicola insolens AT 1.7A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-10-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.635, 50.515, 43.770 |
| Unit cell angles | 90.00, 110.35, 90.00 |
Refinement procedure
| Resolution | 41.170 - 1.660 |
| Rwork | 0.166 |
| R-free | 0.18800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2eng |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.620 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.690 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Rmerge | 0.035 | 0.060 |
| Number of reflections | 20097 | |
| <I/σ(I)> | 41 | 21 |
| Completeness [%] | 99.3 | 96.9 |
| Redundancy | 5.1 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 20% PEG 8000, 0.05M POTASSIUM PHOSPHATE, pH 4.50 |
