1HCJ
Photoproduct of the wild-type Aequorea victoria Green Fluorescent Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-09-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 71.770, 65.670, 110.500 |
| Unit cell angles | 90.00, 103.88, 90.00 |
Refinement procedure
| Resolution | 33.150 - 1.800 |
| R-factor | 0.209 * |
| Rwork | 0.209 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gfl |
| RMSD bond length | 0.018 |
| RMSD bond angle | 0.039 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.150 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.057 | 0.251 |
| Total number of observations | 519063 * | |
| Number of reflections | 92677 | |
| <I/σ(I)> | 4 | 2.7 |
| Completeness [%] | 97.6 | 96.8 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.8 | 4 * | CRYSTALS WERE GROWN AT 4C FROM 50 MM MGCL2, 14-17 % PEG3350 AND 50-100 MM TRIS/CL PH 7.8 - 8.6. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | Tris-Cl | 20 (mM) | pH7.8 |
| 3 | 1 | drop | 0.01 (%(w/v)) | ||
| 4 | 1 | reservoir | 50 (mM) | ||
| 5 | 1 | reservoir | PEG3350 | 14-17 (%(w/v)) | |
| 6 | 1 | reservoir | Tris-Cl | 50-100 (mM) | pH7.8-8.6 |
