1HBU
METHYL-COENZYME M REDUCTASE IN THE MCR-RED1-SILENT STATE IN COMPLEX with COENZYME M
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-05-15 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.100, 116.500, 121.800 |
| Unit cell angles | 90.00, 91.80, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.900 |
| R-factor | 0.162 * |
| Rwork | 0.162 |
| R-free | 0.21100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mro |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 1.920 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.053 | 0.159 |
| Number of reflections | 167654 | |
| <I/σ(I)> | 19.3 | 5.9 |
| Completeness [%] | 94.3 | 61.8 |
| Redundancy | 3.6 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | 4 * | 25% PEG 400, 0.2 M NACL 20 MG/ML FINAL PROTEIN CONC 0.2 M MGCL, 0.1 M HEPES PH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG400 | 20-25 (%) | |
| 2 | 1 | reservoir | 200 (mM) | ||
| 3 | 1 | reservoir | 200 (mM) | ||
| 4 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 5 | 1 | reservoir | glycerol | 20 (%(w/w)) | |
| 6 | 1 | drop | protein | 50 (mg/ml) | |
| 7 | 1 | drop | Tris-HCl | 10 (mM) |
