1HBK

Acyl-CoA binding protein from Plasmodium falciparum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	100
Collection date	1999-11-15
Spacegroup name	P 43
Unit cell lengths	48.665, 48.665, 48.411
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.850 - 2.000
R-factor	0.198
Rwork	0.198
R-free	0.23700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	20.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000 *	1.970
High resolution limit [Å]	2.000 *	2.000 *
Rmerge	0.058 *	0.516 *
Total number of observations	28439 *
Number of reflections	7754 *	736 *
<I/σ(I)>	15.1	1.9
Completeness [%]	99.7	95.3 *
Redundancy	3.2	3.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *	20 *	pH 8.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop	HEPES	50 (mM)
3	1	drop		300 (mM)
4	1	reservoir		10 (mM)
5	1	reservoir	Tris	100 (mM)
6	1	reservoir	PEG2000 MME	20 (%)