1HBH
STRUCTURE OF DEOXYHAEMOGLOBIN OF THE ANTARCTIC FISH PAGOTHENIA BERNACCHII AND STRUCTURAL BASIS OF THE ROOT EFFECT
Experimental procedure
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.650, 96.300, 62.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.200 |
| Rwork | 0.160 |
| R-free | 0.22000 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.300 |
| Phasing software | X-PLOR |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | |
| High resolution limit [Å] | 2.200 | 2.200 * |
| Rmerge | 0.052 * | 0.146 * |
| Total number of observations | 49914 * | |
| Number of reflections | 29393 | |
| Completeness [%] | 79.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.2 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | heamoglobin | 2.9 (%) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | |
| 3 | 1 | reservoir | 100 (mM) | ||
| 4 | 1 | reservoir | IHP | 20 (mM) | |
| 5 | 1 | reservoir | PEG6000 | 6-10 (%(w/v)) |
