1HB8
Structure of bovine Acyl-CoA binding protein in tetragonal crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 173 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-12-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 |
| Unit cell lengths | 47.780, 47.780, 128.810 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.130 - 2.000 |
| R-factor | 0.223 |
| Rwork | 0.223 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2abd |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.066 | 0.292 |
| Number of reflections | 18831 | |
| <I/σ(I)> | 13.8 | 3.6 |
| Completeness [%] | 96.5 | 98.8 |
| Redundancy | 2.7 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 25% PEG5000 MME, 40MM NI(SO4)2, 40MM HEPES PH7.5, pH 7.50 |
