1H98
New Insights into Thermostability of Bacterial Ferredoxins: High Resolution Crystal Structure of the Seven-Iron Ferredoxin from Thermus thermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 29.700, 42.120, 62.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.640 |
| R-factor | 0.159 |
| Rwork | 0.159 |
| R-free | 0.18900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6fd1 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 24.177 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | AMoRE |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 13.400 | 1.730 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.092 | 0.234 |
| Total number of observations | 183792 * | |
| Number of reflections | 9579 | |
| <I/σ(I)> | 5 | 2.4 |
| Completeness [%] | 96.3 | 98.8 |
| Redundancy | 19.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7 * | 2.2 M AMMONIUM SULFATE, 0.1 M SODIUM ACETATE PH 5.0-5.4, 5-10% GLYCEROL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | HEPES | 5 (mM) | pH7.0 |
| 3 | 1 | reservoir | ammonium sulfate | 2.2 (M) | |
| 4 | 1 | reservoir | sodium acetate | 0.1 (M) | pH5.2 |
