1H8L

Duck Carboxypeptidase D Domain II in complex with GEMSA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	110
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 21 3
Unit cell lengths	136.480, 136.480, 136.480
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.030 * - 2.600
R-factor	0.199
Rwork	0.199
R-free	0.23900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qmu
RMSD bond length	0.007
RMSD bond angle	1.340
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.030 *	2.740
High resolution limit [Å]	2.600	2.600
Rmerge	0.083	0.475
Total number of observations	362074 *
Number of reflections	26360
<I/σ(I)>	7.8	1.6
Completeness [%]	100.0	100
Redundancy	13.7	13.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	8 *	20 *	Gomis-Rueth, F.X., (1999) Embo J., 18, 5817. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris-HCl	5 (mM)
3	1	drop		50 (mM)
4	1	drop	benzamidine	0.5 (mM)
5	1	reservoir	ammonium sulfate	2.1 (M)
6	1	reservoir	sodium acetate	0.1 (M)