1H84
COVALENT ADDUCT BETWEEN POLYAMINE OXIDASE AND N1ethylN11((cycloheptyl)methyl)4,8diazaundecane at pH 4.6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-3 |
Synchrotron site | ESRF |
Beamline | ID14-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 184.040, 184.040, 280.800 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.000 |
R-factor | 0.189 |
Rwork | 0.189 |
R-free | 0.22700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1b37 |
RMSD bond length | 0.009 |
RMSD bond angle | 2.100 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CCP4 |
Refinement software | TNT (5D) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.070 | 0.214 |
Total number of observations | 617266 * | |
Number of reflections | 172552 | |
Completeness [%] | 93.5 | 90.8 |
Redundancy | 3.2 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6 * | 20 * | pH 4.60 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | enzyme | 4 (mg/ml) | |
2 | 1 | drop | 300 (mM) | ||
3 | 1 | drop | sodium phosphate | 50 (mM) | |
4 | 1 | reservoir | ammonium sulfate | 42-50 (%sat) | |
5 | 1 | reservoir | sodium acetate | 100 (mM) |