1H7P
SCHIFF-BASE COMPLEX OF YEAST 5-AMINOLAEVULINIC ACID DEHYDRATASE WITH 4-KETO-5-AMINO-HEXANOIC (KAH) AT 1.64 A RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-06-15 |
| Detector | MARRESEARCH |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 104.300, 104.300, 167.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.000 - 1.640 |
| R-factor | 0.249 |
| R-free | 0.30100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ylv |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.023 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CCP4 |
| Refinement software | SHELX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 1.730 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.084 | 0.409 |
| Number of reflections | 56412 | |
| <I/σ(I)> | 5.4 | 1.8 |
| Completeness [%] | 99.3 | 96.9 |
| Redundancy | 6.9 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8 | Erskine, P.T., (1997) Protein Sci., 6, 1774. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 2 (mg/ml) | |
| 2 | 1 | reservoir | Tris-HCl | 0.2 (M) | |
| 3 | 1 | reservoir | PEG6000 | 50 (%(w/v)) | 0.040ml-0.240ml |
| 4 | 1 | reservoir | zinc sulfate | 4mg | |
| 5 | 1 | reservoir | beta-mercaptoethanol | 0.100ml in 10ml water |
