1H7I
Apolipoprotein E3 22kD fragment LYS146GLN mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 120 |
| Collection date | 1995-03-15 |
| Detector | ADSC ADSC MULTIWIRE |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.700, 52.960, 84.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.205 |
| Rwork | 0.203 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bz4 |
| RMSD bond length | 0.037 |
| Data reduction software | ADSC |
| Data scaling software | ADSC |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.041 | 0.247 |
| Number of reflections | 14706 | |
| <I/σ(I)> | 22.4 | 2 |
| Completeness [%] | 98.0 | 96.6 |
| Redundancy | 3.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 50MM NA-CACODYLATE, PH 5.6, 20-25% PEG 400, 1% 2-ME. RT. ORTHORHOMBIC FORM ORTHO-2 APPEARS (SEE PDB ENTRY 1OR2). |
