1H7C

human tubulin chaperone cofactor a

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2000-02-15
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	C 1 2 1
Unit cell lengths	99.875, 22.793, 46.845
Unit cell angles	90.00, 101.10, 90.00

Refinement procedure

Resolution	37.300 * - 2.000 *
R-factor	0.21 *
Rwork	0.210
R-free	0.26200 *
Structure solution method	MAD
RMSD bond length	0.005 *
RMSD bond angle	1.000 *
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.300 *	2.000
High resolution limit [Å]	2.000 *	2.000 *
Rmerge	0.040 *	0.081 *
Total number of observations	25086 *
Number of reflections	7295 *
Completeness [%]	99.9 *	99.9 *
Redundancy	3.4 *	2.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.6 *	20 *	pH 6.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	MES	20 (mM)	pH6.6
3	1	reservoir	ammonium sulfate	2 (M)	pH4.9
4	1	reservoir		0.1 (M)