1H6O
Dimerisation domain from human TRF1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-03-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 85.390, 85.390, 91.630 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.840 - 2.900 |
| R-factor | 0.25 |
| Rwork | 0.250 |
| R-free | 0.30300 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 18.600 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CCP4 |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.690 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.057 | 0.140 |
| Total number of observations | 45019 * | |
| Number of reflections | 8514 | |
| <I/σ(I)> | 8.4 | 5 |
| Completeness [%] | 97.1 | 97.1 |
| Redundancy | 5.3 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 9 * | PH6, 5-15% GLYCEROL, 1-5 MM MAGNESIUM ACETATE, 1 % PEG 8000, pH 6.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 10 (mM) | |
| 10 | 1 | reservoir | PEG8000 | 1 (%) | |
| 2 | 1 | drop | 200 (mM) | ||
| 3 | 1 | drop | glycerol | 10 (%) | |
| 4 | 1 | drop | PMSF | 0.5 (mM) | |
| 5 | 1 | drop | mercaptoethanol | 10 (mM) | |
| 6 | 1 | drop | protein | 34 (mg/ml) | |
| 7 | 1 | reservoir | sodium cacodylate | 100 (mM) | |
| 8 | 1 | reservoir | glycerol | 5-15 (%) | |
| 9 | 1 | reservoir | 1-5 (mM) |
