1H6A
Reduced Precursor Form of Glucose-Fructose Oxidoreductase from Zymomonas mobilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 83.896, 93.060, 115.486 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.500 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| R-free | 0.27500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ofg |
| RMSD bond length | 0.008 |
| RMSD bond angle | 23.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.630 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.120 | 0.385 |
| Number of reflections | 31238 | |
| <I/σ(I)> | 4.2 | 1.9 |
| Completeness [%] | 97.9 | 97.3 |
| Redundancy | 3.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.4 * | 12% PEG6000, 210MM AMMONIUM SULFATE, 20% GLYCEROL, 100MM K-SUCCINATE PH6.5, pH 6.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | MES/KOH | 40 (mM) | pH6.4 |
| 3 | 1 | reservoir | PEG6000 | 12 (%) | |
| 4 | 1 | reservoir | glycerol | 20 (%) | |
| 5 | 1 | reservoir | ammonium sulfate | 210 (mM) | |
| 6 | 1 | reservoir | potassium succinate | 100 (mM) | pH5.5 |
