1H68
sensory rhodopsin II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Collection date | 2000-10-15 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 84.830, 128.740, 50.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.900 - 2.100 |
| R-factor | 0.237 |
| Rwork | 0.237 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qhj |
| RMSD bond length | 0.006 |
| RMSD bond angle | 17.500 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.900 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.072 | 0.425 |
| Total number of observations | 99617 * | |
| Number of reflections | 15956 | |
| <I/σ(I)> | 8.4 | 1.8 |
| Completeness [%] | 96.1 | 80.1 |
| Redundancy | 6.3 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 4.6 | pH 4.60 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 4.5 (mg/ml) | |
| 2 | 1 | 1 | n-dodecyl beta-D-maltoside | 0.005 (%) | |
| 3 | 1 | 1 | 75 (mM) | ||
| 4 | 1 | 1 | 1.5 (M) |
