1H5W

2.1A Bacteriophage Phi-29 Connector

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	1999-11-21
Detector	ADSC CCD
Spacegroup name	I 4 2 2
Unit cell lengths	155.340, 155.340, 160.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.000 - 2.100
R-factor	0.205
Rwork	0.205
R-free	0.24700 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fou
RMSD bond length	0.006
RMSD bond angle	1.130 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	DM
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.000	2.210
High resolution limit [Å]	2.100	2.100
Rmerge	0.100	0.400
Total number of observations	327940 *
Number of reflections	56472 *
<I/σ(I)>	5.4	2.1
Completeness [%]	98.9	94.1
Redundancy	5.8	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	8	20 *	70% MPD, 0.1M TRIS-HCL PH 8

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	MPD	70 (%)
2	1	reservoir	Tris-HCl	0.1 (M)	pH8.0