1H4U

Domain G2 of mouse nidogen-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	CCD
Collection date	2000-08-18
Detector	QUANTUM4
Spacegroup name	P 64
Unit cell lengths	95.180, 95.180, 65.390
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 2.200
R-factor	0.241
Rwork	0.241
R-free	0.28000
Structure solution method	MIRAS
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	MOSFLM
Data scaling software	CCP4
Phasing software	CCP4
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.066	0.391
Number of reflections	17203
<I/σ(I)>	14.4	3.8
Completeness [%]	99.7	100
Redundancy	5	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.8 *		10 MG/ML PROTEIN, 5-8% PEG4000, 0.1 M ACETATE PH 5.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	ammonium carbonate	0.01 (M)
3	1	reservoir	PEG4000	5-8 (%(w/v))
4	1	reservoir	sodium acetate	0.1 (M)