1H4U
Domain G2 of mouse nidogen-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-08-18 |
| Detector | QUANTUM4 |
| Spacegroup name | P 64 |
| Unit cell lengths | 95.180, 95.180, 65.390 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.200 |
| R-factor | 0.241 |
| Rwork | 0.241 |
| R-free | 0.28000 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | CCP4 |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.066 | 0.391 |
| Number of reflections | 17203 | |
| <I/σ(I)> | 14.4 | 3.8 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.8 * | 10 MG/ML PROTEIN, 5-8% PEG4000, 0.1 M ACETATE PH 5.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | ammonium carbonate | 0.01 (M) | |
| 3 | 1 | reservoir | PEG4000 | 5-8 (%(w/v)) | |
| 4 | 1 | reservoir | sodium acetate | 0.1 (M) |
