1H3Q

Crystal structure of SEDL at 2.4 Angstroms resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 6B
Synchrotron site	PAL/PLS
Beamline	6B
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MACSCIENCE
Wavelength(s)	0.97934,0.97947,0.97166
Spacegroup name	C 2 2 21
Unit cell lengths	46.690, 101.300, 66.150
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.400
R-factor	0.213
Rwork	0.213
R-free	0.24800
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.236
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.400
High resolution limit [Å]	2.400	2.300
Rmerge	0.066 *	0.146 *
Total number of observations	33601 *
Number of reflections	6083
<I/σ(I)>	24.2	6
Completeness [%]	97.3	94.7
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5	298 *	1.4M SODIUM ACETATE, 0.1M SODIUM CACODYLATE PH6.5, pH 6.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	50 (mM)	pH7.6
2	1	drop		250 (mM)
3	1	drop	protein	10 (mg/ml)
4	1	reservoir	sodium acetate	1.4 (M)
5	1	reservoir	sodium cacodylate	0.1 (M)	pH6.5