1H3L

N-terminal fragment of SigR from Streptomyces coelicolor

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID13
Synchrotron site	ESRF
Beamline	ID13
Temperature [K]	100
Detector technology	CCD
Collection date	1999-09-15
Detector	MARRESEARCH
Spacegroup name	P 32 2 1
Unit cell lengths	71.546, 71.546, 102.794
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.000 - 2.400 *
R-factor	0.25939
Rwork	0.258
R-free	0.27800 *
Structure solution method	MAD
RMSD bond length	0.029 *
RMSD bond angle	2.440 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.047 *	0.142 *
Number of reflections	12713
<I/σ(I)>	25.2	5.3
Completeness [%]	97.9 *	83.4 *
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5	4 *	10-15%(W/V) PEG 8000 IN 100MM TRIS-HCL PH8.5, pH 8.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG8000	10-15 (%(w/v))
3	1	reservoir	Tris-HCl	100 (mM)	pH8.5
4	1	reservoir	dithiothreitol	1 (mM)