1H32

Reduced SoxAX complex from Rhodovulum sulfidophilum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.2
Synchrotron site	SRS
Beamline	PX14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-05-15
Detector	ADSC CCD
Spacegroup name	P 21 21 2
Unit cell lengths	65.795, 87.186, 71.459
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 1.500
R-factor	0.199
Rwork	0.199
R-free	0.23000 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.120
RMSD bond angle	1.316
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000
High resolution limit [Å]	1.500
Rmerge	0.041	0.284 *
Total number of observations	670264 *
Number of reflections	66141
Completeness [%]	99.3	98.4 *
Redundancy	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.5		pH 6.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	15 (%(w/v))
2	1	reservoir	magnesium acetate	0.2 (M)
3	1	reservoir	MES	100 (mM)	pH6.5