1H2K
Factor Inhibiting HIF-1 alpha in complex with HIF-1 alpha fragment peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-05-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 86.161, 86.161, 146.656 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.500 - 2.150 |
| R-factor | 0.18 |
| Rwork | 0.178 |
| R-free | 0.21300 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.374 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.170 | 2.270 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.052 | 0.331 |
| Number of reflections | 30574 | |
| <I/σ(I)> | 9.9 | 1.5 |
| Completeness [%] | 99.2 | 96 |
| Redundancy | 6.3 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | 17 * | 1.2M AMMONIUM SULPHATE, 4% PEG400, 0.1M HEPES PH7.5, ARGON ATMOSPHERE, 11MG/ML PROTEIN WITH 1MM FE(II), 2.5MM NOG AND 2.5MM PEPTIDE, pH 7.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 1.2 (M) | |
| 2 | 1 | reservoir | PEG400 | 4 (%) | |
| 3 | 1 | reservoir | HEPES | 0.1 (M) | pH7.5 |
| 4 | 1 | drop | FIH | 11 (mg/ml) | |
| 5 | 1 | drop | Fe2+ | 1 (mM) | |
| 6 | 1 | drop | 2OG/NOG | 2 (mM) | |
| 7 | 1 | drop | CAD fragment | 1 (mM) |
