1H2I

Human Rad52 protein, N-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Spacegroup name	P 1 21 1
Unit cell lengths	117.259, 127.319, 191.227
Unit cell angles	90.00, 90.29, 90.00

Refinement procedure

Resolution	30.000 - 2.700
R-factor	0.225
Rwork	0.225
R-free	0.26200
Structure solution method	MIRAS
RMSD bond length	0.014
RMSD bond angle	1.780
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.055	0.279
Number of reflections	149457
<I/σ(I)>	14	2.7
Completeness [%]	97.0	96.7
Redundancy	2.3	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *		pH 8.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris-HCl	10 (mM)	pH7.5
3	1	drop		300 (mM)
4	1	drop	dithiothreitol	1 (mM)
5	1	reservoir	Tris-HCl	100 (mM)	pH8.0
6	1	reservoir	ethanol	4-14 (%(v/v))