1H2D

Ebola virus matrix protein VP40 N-terminal domain in complex with RNA (Low-resolution VP40[31-212] variant).

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Spacegroup name	P 62 2 2
Unit cell lengths	79.610, 79.610, 239.180
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	59.800 * - 2.600
R-factor	0.306
Rwork	0.305
R-free	0.32900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h2c
RMSD bond length	0.009 *
RMSD bond angle	1.420 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	BEAST
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.000
High resolution limit [Å]	2.600
Rmerge	0.085	0.406 *
Total number of observations	63464 *
Number of reflections	14356 *
<I/σ(I)>	4.5
Completeness [%]	97.8	86 *
Redundancy	4.4	3.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5 *		1 UL OF PROTEIN (13 MG/ML) AND 1 UL OF WELL SOLUTION (100 MM NA-ACETATE PH4.6, 35 % MPD, 4 % GLYCEROL).HANGING DROP., pH 4.60

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	HEPES	100 (mM)	pH7.5
3	1	reservoir		1.5 (M)
4	1	reservoir	MPD	15 (%)