1H1Y
The structure of the cytosolic D-ribulose-5-phosphate 3-epimerase from rice complexed with sulfate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Temperature [K] | 100 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.280, 73.300, 88.970 |
| Unit cell angles | 90.00, 101.75, 90.00 |
Refinement procedure
| Resolution | 29.000 * - 1.900* |
| R-factor | 0.175 |
| Rwork | 0.175 |
| R-free | 0.20600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.000 | |
| High resolution limit [Å] | 1.900 * | |
| Rmerge | 0.021 | 0.042 * |
| Number of reflections | 42044 | 5730 * |
| Completeness [%] | 98.0 | 98 * |
| Redundancy | 4.5 | 4.3 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | pH 7.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 1.0 (M) | |
| 3 | 1 | drop | MOPS | 50 (mM) | pH7.5 |
| 4 | 1 | drop | PEG400 | 1 (%(w/v)) | |
| 5 | 1 | drop | glycerol-3-phosphate | 10 (mM) | |
| 6 | 1 | drop | L-arginine | 5 (mM) | |
| 7 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
| 8 | 1 | reservoir | MOPS | 100 (mM) | pH7.5 |
| 9 | 1 | reservoir | PEG400 | 2 (%) |
