1H19
STRUCTURE OF [E271Q]LEUKOTRIENE A4 HYDROLASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-04-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 77.962, 86.873, 98.787 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.160 - 2.100 |
| R-factor | 0.184 |
| Rwork | 0.184 |
| R-free | 0.23300 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hs6 |
| RMSD bond length | 0.010 * |
| RMSD bond angle | 1.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.080 | 0.186 |
| Number of reflections | 62349 | |
| <I/σ(I)> | 10.3 | |
| Completeness [%] | 99.6 | 99.3 |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | liquid-liquid diffusion * | 6.8 | PEG8000, IMIDAZOLE, SODIUM ACETATE, YTTERBIUM CHLORIDE, BESTATIN, pH 6.80 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | PEG8000 | 28 (%(v/v)) | |
| 2 | 1 | 1 | sodium acetate | 0.1 (mM) | |
| 3 | 1 | 1 | imidazole | 0.1 (mM) | pH6.8 |
| 4 | 1 | 1 | 5 (mM) | ||
| 5 | 1 | 2 | LTA4H | 5 (mg/ml) | |
| 6 | 1 | 2 | Tris-HCl | 10 (mM) | pH8. |
