1H10
HIGH RESOLUTION STRUCTURE OF THE PLECKSTRIN HOMOLOGY DOMAIN OF PROTEIN KINASE B/AKT BOUND TO INS(1,3,4,5)-TETRAKISPHOPHATE
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-01-15 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 82.868, 34.351, 44.293 |
| Unit cell angles | 90.00, 115.31, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.400 |
| R-factor | 0.125 * |
| R-free | 0.17500 * |
| Structure solution method | DIRECT METHODS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.030 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.055 | 0.231 |
| Total number of observations | 83520 * | |
| Number of reflections | 21933 | |
| <I/σ(I)> | 30.8 | 6.7 |
| Completeness [%] | 97.8 | 83.3 |
| Redundancy | 3.8 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.6 * | 0.25M AMMONIUM ACETATE, 30% PEG 4000,0.1M SODIUM ACETATE (PH 4.6) |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8.5 (mg/ml) | |
| 2 | 1 | reservoir | ammonium acetate | 0.25 (M) | |
| 3 | 1 | reservoir | PEG4000 | 30 (%) | |
| 4 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.6 |
