1H0P
Cyclophilin_5 from C. elegans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 61 |
| Unit cell lengths | 94.115, 94.115, 41.239 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.500 * - 1.750 |
| R-factor | 0.236 * |
| R-free | 0.16900 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e8k |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.023 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.500 * | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.086 | 0.530 |
| Number of reflections | 21525 | |
| <I/σ(I)> | 27.7 | 3.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 9.5 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 | 18 * | PH 6.0, PEG 4000, LITHIUM CHLORIDE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10.2 (mg/ml) | |
| 2 | 1 | drop | Tris | 50 (mM) | |
| 3 | 1 | drop | 300 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 5 (mg/ml) | |
| 5 | 1 | reservoir | sodium citrate | 100 (mM) | pH6.0 |
| 6 | 1 | reservoir | PEG4000 | 34 (%(w/v)) | |
| 7 | 1 | reservoir | 20-40 (mM) |
