1H0H
Tungsten containing Formate Dehydrogenase from Desulfovibrio Gigas
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-11-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.992,1.214,1.735,1.7415 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.408, 110.398, 156.185 |
| Unit cell angles | 90.00, 93.53, 90.00 |
Refinement procedure
| Resolution | 35.000 - 1.800 |
| R-factor | 0.169 |
| Rwork | 0.167 |
| R-free | 0.21300 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nap |
| RMSD bond length | 0.019 * |
| RMSD bond angle | 1.792 * |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.880 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.078 | 0.600 |
| Total number of observations | 208782 * | |
| Number of reflections | 10979 * | |
| <I/σ(I)> | 5 | 2 |
| Completeness [%] | 92.0 | 70 |
| Redundancy | 1.9 | 1.65 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7.5 | 4 * | used macroseeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES | 0.1 (M) | pH7.5 |
| 2 | 1 | drop | PEG3350 | 20 (%(w/v)) | |
| 3 | 1 | drop | isopropanol | 10 (%(v/v)) | |
| 4 | 1 | drop | beta-octylglucoside | 1 (%) |
