1H0B

Endoglucanase cel12A from Rhodothermus marinus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	291
Detector technology	IMAGE PLATE
Collection date	2001-01-15
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	56.099, 67.779, 132.262
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 * - 1.800
R-factor	0.173
Rwork	0.173
R-free	0.19400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1nlr
RMSD bond length	0.005
RMSD bond angle	1.340 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.074	0.275
Total number of observations	308104 *
Number of reflections	44464
<I/σ(I)>	27.7	5.5
Completeness [%]	93.4	88.1
Redundancy	6.9	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5	291 *	0.1M HEPES, PH7.5, 20% PEG10000, pH 7.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	14 (mg/ml)
2	1	reservoir	HEPES	0.1 (M)	pH7.5
3	1	reservoir	PEG10000	20 (%(w/v))