1H09
Multimodular Pneumococcal Cell Wall Endolysin from phage Cp-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-05-15 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 77.950, 95.780, 129.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.100 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.25900 * |
| Structure solution method | OTHER |
| RMSD bond length | 0.005 * |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.000 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.073 | 0.349 |
| Number of reflections | 28359 | |
| <I/σ(I)> | 6.8 | 2 |
| Completeness [%] | 99.3 | 99.3 |
| Redundancy | 4.7 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * | 20 * | 1.7M NA FORMATE, 0.1M NA CIT.(PH6),1.8MM N-DECYL-MALTOSIDE, pH 6.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 20 (mM) | pH8.0 |
| 2 | 1 | drop | protein | 8 (mg/ml) | |
| 3 | 1 | reservoir | sodium formate | 1.7 (M) | |
| 4 | 1 | reservoir | sodium citrate | 0.1 (M) | pH6.0 |
| 5 | 1 | reservoir | n-decyl-beta-D-maltoside | 1.8 (mM) |
