1GZV
The crystal structure of phosphoglucose isomerase from pig muscle complexed with 5-phosphoarabinonate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ELLIOT |
| Temperature [K] | 294 |
| Detector technology | DIFFRACTOMETER |
| Detector | HILGER-WATTS |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 95.600, 95.600, 138.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 3.510 |
| Rwork | 0.190 |
| R-free | 0.24900 |
| Structure solution method | OTHER |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.974 |
| Refinement software | REFMAC (5.0.32) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 17.700 |
| High resolution limit [Å] | 3.500 |
| Rmerge | 0.045 |
| Number of reflections | 7817 |
| Completeness [%] | 92.8 |
| Redundancy | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 277 | THE PROTEIN WAS CONCENTRATED TO 10MG/ML AND CRYSTALLIZED AT 4 DEGRESS CELSIUS FROM 10MM SODIUM PHOSPHATE, PH 7.5 AND 57% SATURATED AMMONIUM SULPHATE. |
