1GZA

PEROXIDASE

Experimental procedure

Source type	ROTATING ANODE
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 42 21 2
Unit cell lengths	74.570, 74.570, 117.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 - 2.060
R-factor	0.162
Rwork	0.162
RMSD bond length	0.021
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.060 *
Rmerge	0.062 *
Total number of observations	125031 *
Number of reflections	20178 *
Completeness [%]	95.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *	22-26 *	repeated seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	ARP	20-40 (mg/ml)
2	1	reservoir	ammonium sulfate	32 (%sat)
3	1	reservoir	Tris-HCl	20 (mM)