1GZ7
Crystal structure of the closed state of lipase 2 from Candida rugosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2001-05-15 |
| Spacegroup name | P 1 |
| Unit cell lengths | 61.150, 91.140, 108.460 |
| Unit cell angles | 90.78, 106.31, 86.91 |
Refinement procedure
| Resolution | 12.000 * - 1.970 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.23000 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1trh |
| RMSD bond length | 0.006 |
| RMSD bond angle | 23.200 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.110 | 2.080 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.100 * | 0.300 * |
| Total number of observations | 299637 * | |
| Number of reflections | 152503 | |
| <I/σ(I)> | 4.6 | 2.2 |
| Completeness [%] | 94.6 | 76.5 |
| Redundancy | 2 | 1.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5 * | 291 * | 15% (W/V) PEG 4000, SODIUM ACETATE 0.1M, PH 5.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 15 (%(w/v)) | |
| 2 | 1 | reservoir | sodium acetate | 0.1 (M) | pH5.0 |
| 3 | 1 | drop | protein | 7-8 (mg/ml) |
