1GYX
The Crystal Structure of YdcE, a 4-Oxalocrotonate Tautomerase Homologue from Escherichia coli, Confirms the Structural Basis for Oligomer Diversity
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 2000-10-15 |
Detector | RIGAKU IMAGE PLATE |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.566, 46.525, 75.724 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 1.350 |
Rwork | 0.142 |
R-free | 0.16400 |
Structure solution method | MIR |
RMSD bond length | 0.009 * |
RMSD bond angle | 1.500 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.390 |
High resolution limit [Å] | 1.350 | 1.350 |
Rmerge | 0.036 | 0.175 |
Total number of observations | 156715 * | |
Number of reflections | 35377 | |
<I/σ(I)> | 24.7 | 3.8 |
Completeness [%] | 87.4 | 33 |
Redundancy | 2.5 | 1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8.5 * | 4 * | PROTEIN: 25MG/ML IN 50MM HEPES, PH 7.3, 4 CELCIUS SITTING DROP: MIX 15UL PROTEIN WITH 5UL 40% SODIUM CITRATE PH 6.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | reservoir | sodium citrate | 40 (%(w/v)) | pH8.5 |