1GYW
Gamma-adaptin appendage domain from clathrin adaptor AP1 A753D mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-07-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 62.155, 62.155, 148.903 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.740 - 2.400 |
| Rwork | 0.190 |
| R-free | 0.26871 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | WILD-TYPE 1GYU |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.060 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.086 | 0.330 |
| Number of reflections | 11979 | |
| <I/σ(I)> | 8.1922 | 2.18 |
| Completeness [%] | 99.3 | 96.3 |
| Redundancy | 13.62 | 13.41 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 | 24% PEG4000, 100 MM TRIS PH 8.0, 200MM MGCL2, VAPOUR DIFFUSION |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES | 5 (mM) | |
| 2 | 1 | drop | 50 (mM) | ||
| 3 | 1 | drop | dithiothreitol | 4 (mM) | |
| 4 | 1 | drop | protein | 5 (mg/ml) | |
| 5 | 1 | reservoir | PEG4000 | 24 (%) | |
| 6 | 1 | reservoir | Tris | 100 (mM) | pH8.0 |
| 7 | 1 | reservoir | 200 (mM) |
