1GYV
Gamma-adaptin appendage domain from clathrin adaptor AP1, L762E mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-06-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 33.720, 54.820, 67.480 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.640 - 1.700 * |
| R-factor | 0.182 |
| Rwork | 0.178 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gyu |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.900 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.700 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.062 | 0.279 |
| Number of reflections | 14209 | |
| <I/σ(I)> | 22 | 6 |
| Completeness [%] | 99.6 | 97.9 |
| Redundancy | 6.4 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.4 | 50MM HEPES PH7.4,0.9M NA/K TARTRATE 20% W/V GLYCEROL, pH 7.40 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES | 5 (mM) | |
| 2 | 1 | drop | 50 (mM) | ||
| 3 | 1 | drop | dithiothreitol | 4 (mM) | |
| 4 | 1 | drop | protein | 5 (mg/ml) | |
| 5 | 1 | reservoir | HEPES | 50 (mM) | pH7.4 |
| 6 | 1 | reservoir | sodium acetate | 0.9 (M) | |
| 7 | 1 | reservoir | glycerol | 20 (%(w/v)) |
