1GYN
Class II fructose 1,6-bisphosphate aldolase with Cadmium (not Zinc) in the active site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Detector | FUJI |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 77.331, 77.331, 290.851 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.300 * - 2.000 |
| R-factor | 0.169 |
| Rwork | 0.168 |
| R-free | 0.19300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zen |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.300 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.060 * | 0.290 * |
| Total number of observations | 102727 * | |
| Number of reflections | 31355 | |
| <I/σ(I)> | 12.8 | 1.3 |
| Completeness [%] | 86.9 | 51 |
| Redundancy | 2.5 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 7.5 * | pH 7.60 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | enzyme | 16.5 (mg/ml) | |
| 2 | 1 | 1 | Tris-HCl | 50 (mM) | pH7.5 |
| 3 | 1 | 1 | PEG4000 | 3-4 (%(w/v)) | |
| 4 | 1 | 1 | 2 (mM) |
