1GYM

PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE C IN COMPLEX WITH GLUCOSAMINE-(ALPHA-1-6)-MYO-INOSITOL

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1995-07-22
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	P 21 21 21
Unit cell lengths	45.200, 46.500, 161.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.200
R-factor	0.178
Rwork	0.178
R-free	0.27200
RMSD bond length	0.015
RMSD bond angle	24.800 *
Data reduction software	DETECTOR (SUPPLIED SOFTWARE)
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.200	2.200 *
Rmerge	0.047 *
Total number of observations	32130 *
Number of reflections	15359
Completeness [%]	75.0 *	62.2 *
Redundancy	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	PI-PLC	4 (mg/ml)
2	1	drop	GlcN(alpha1-6)Ins.	15 (mM)
3	1	drop	HEPES	20 (mM)
4	1	drop		0.005 (%)
5	1	drop	PEG600	18.5 (%)
6	1	drop	trisodium citrate	0.095 (M)
7	1	drop	beta-mercaptoethanol	1.25 (mM)
8	1	reservoir		2.25 (M)